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Protein protein interaction
Protein-protein interactions (PPIs) play a crucial role in cellular functions and biological processes in all organisms. The identification of protein interactions can lead to a better understanding of infection mechanisms and the development of several medication drugs and treatment optimization. Among these associations, protein-protein interactions are particularly important due to their versatility, specificity and adaptability. The STRING database aims to integrate all known and predicted associations between proteins, including both physical interactions as well as functional associations. The pull-down assay is an in vitro method used to determine a physical interaction between two or more proteins. Immunoprecipitation (IP), a method using a target protein-specific antibody in conjunction with Protein A/G affinity beads, is a powerful tool to identify molecules that interact with specific proteins. A vast network of genes is inter-linked through protein-protein interactions and is critical component of almost every biological process under physiological conditions. Any disruption of the biologically essential network leads to pathological conditions resulting into related diseases. Protein-protein interaction plays key role in predicting the protein function of target protein and drug ability of molecules. The majority of genes and proteins realize resulting phenotype functions as a set of interactions
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